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发明公开
CN101463359A 融合免疫毒素B-L-Vpr基因及克隆和异源表达方法 无效 - 撤回

融合免疫毒素B-L-Vpr基因及克隆和异源表达方法
摘要：
本发明涉及基因工程领域，具体的说是一种融合免疫毒素B－L－Vpr基因及克隆和异源表达方法。具有序列表SEQ ID No：1中碱基序列，同时融合免疫毒素B－L－Vpr基因所编码的蛋白具有SEQ ID No：2中氨基酸序列。本发明以抗HER－2单链抗体作为靶向分子，以Vpr蛋白作为效应分子，经短肽连接构建成融合免疫毒素B－L－Vpr，并在大肠杆菌中高效表达融合免疫毒素蛋白。该融合免疫毒素可对HER－2受体过表达的肿瘤细胞产生特异性的细胞抑制作用。
摘要（英）：

The invention relates to the field of genetic engineering, in particular to a fusion immunotoxin B-L-Vpr gene as well as cloning and heterologous expression. The gene has a base sequence in Sequence Table SEQ ID No:1, meanwhile, protein encoded by the fusion immunotoxin B-L-Vpr gene has an amino acid sequence in SEQ ID No:2. In the invention, a single-chain antibody for resisting HER-2 is regarded as a targeted molecule and Vpr protein is regarded as an effector molecule. The targeted molecule and the effector molecule form the fusion immunotoxin B-L-Vpr through the connection of small peptides and effectively express the fusion immunotoxin protein in colon bacillus. The fusion immunotoxin can have a specific inhibitive effect on a tumor cell over-expressed by a HER-2 receptor.

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