The invention discloses a technetium-99m labeled 6-hydrazino-pyridine-3-formyl amino lactose albumin new complex, a preparation method and application thereof. The complex takes N-hydroxyethyl-ethylenediamine-N, N', N'-triacetic acid (HEDTA) as a co-ligand, and under the presence of co-ligand and reducing agent, the radioactive technetium-99m labeled 6-hydrazino-pyridine-3-formyl amino lactose albumin new complex is prepared by reaction when the 6-hydrazino-pyridine-3-formyl amino new lactose albumin is matched. The complex has good chemical stability and biological property, high initial uptake value and target and non-target ratio, simple preparation and low use cost, and can be used in radiopharmaceuticals and clinical nuclear medicine technology field as a novel liver imaging agent.