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发明授权
CN101597612B 一种超抗原活性增强的SEC2突变基因及其制备方法 有权

一种超抗原活性增强的SEC2突变基因及其制备方法
摘要：
本发明涉及基因工程技术，具体说是一种超抗原活性增强的SEC2突变基因及其制备方法。具体为具有序列表SEQ ID NO：1中的碱基序列并具有序列表SEQ ID NO：2中的氨基酸序列。本发明利用基因定点突变技术，首次构建了超抗原活性和抗肿瘤效应显著增强的SEC2突变蛋白，并成功在大肠杆菌中实现可溶性表达。本发明可提供直接用于生产该超抗原突变蛋白的基因工程菌株。
摘要（英）：

The invention relates to a gene engineering technology, in particular to an SEC2 mutant gene with increased activity of super-antigen and a preparation method thereof. The SEC2 mutant gene is provided with a base sequence in a sequence table SEQ ID NO:1 and also provided with an amino acid sequence in a sequence table SEQ ID NO:2. The invention establishes the SEC2 mutant gene with remarkably enhanced super-antigen activity and antitumor effect for the first time by using a gene targeted mutagenesis technology and successfully realizes the soluble expression in escherichia coli. The inventioncan provide the gene engineering strain directly used for producing the mutant protein of the super-antigen.

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