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CN101624625A 食品中常见弯曲菌检测试剂盒及其检测方法 失效 - 权利终止 转让

食品中常见弯曲菌检测试剂盒及其检测方法
摘要：
一种用于食品微生物污染检测及监控的食品中常见弯曲菌检测试剂盒及其检测方法。该试剂盒中，1mL检测溶液含10mM Tris·Cl、50mM KCl、25mMMgCl 2 、dNTP(dATP、dGTP、dCTP和dTTP)各2.5mM、Taq DNA聚合酶5U/μL及四种常见弯曲菌引物对各10μM；本方法采用弯曲菌的毒力因子基因检测，可以在检测出弯曲菌的同时进行四种类型的区分；检测时间仅1.5天，比传统微生物学方法检测时间大为缩短；检测限低于15cfu/mL，只要每毫升菌液里各弯曲菌数不低于15个即可检出。并且本方法检侧时间比传统方法短，方法更加简捷，可以节省大量的劳力和财力，适合快速检测的要求。
摘要（英）：

The invention relates to a detection kit for common campylobacter in foods and a detection method thereof, which is used for detecting and monitoring the microbial contamination of the foods. In the kit, 1mL of detection solution contains 10mM of Tris.Cl, 50mM of KCl, 25mM of MgCl2, 2.5mM of dNTP (dATP, dGTP, dCTP and dTTP) respectively, 5U/mu L of Taq DNA polymerase and 10muM of four kinds of common campylobacter primer pairs; the method adopts the virulence factor genes of the campylobacter to carry out detection, and can simultaneously differentiate four types when detecting the campylobacter; the detection time is only 1.5 days and is greatly shortened in comparison with the detection time of a traditional microbiology method; and the detection limit is below 15cfu/mL, and the campylobacter can be detected so long as the number of the campylobacter in bacterium liquid per milliliter is not less than 15. Moreover, the method has shorter detection time in comparison with the traditional method, is simpler and quicker, can save a large number of labour force and resources and is suitable for the requirement of quick detection.

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