The invention relates to a high-efficient extraction and purification method of rape lipase, which comprises the following steps: (1) determining the optimal time for extracting the lipase from rape; (2) adding solid ammonium sulfate in the extracted crude lipase liquid under stirring, precipitating proteins, and using distilled water for dissolving, thereby obtaining salting-out solution, wherein the recovery rate of the lipase is 93.49% and the purification fold achieves 1.06; (3) then selecting an ultrafiltration membrane with the intercepted molecular weight of 100000Da for concentrating the salting-out solution of the lipase; and (4) adopting the ion exchange chromatography for purifying the lipase in ultra-filtrate, thereby obtaining rape lipase proteins, wherein the purity is 3.13u/mg and 9.14u/mg respectively, and the overall recovery rate is 43.94%. The purification process of the rape lipase provided by the invention is different from the separation and purification technology of microbial lipase, and liquid nitrogen is adopted for grinding and breaking plant cell walls, thereby dissolving the lipase in plant cytoplasms and the lipase in plant plasma membranes. Thus, theinvention provides the method for extracting and purifying the lipase secreted in plant cells, which has the advantages of high purity, high recovery rate and enzyme purification fold of 4.83.