The invention discloses health-care vinegar, a brewing method and application in preventing cardiovascular diseases thereof. The method comprises the following steps: (1) removing peel of 6 to 7 weight percent of fresh kudzuvine root after the fresh kudzuvine root is cleaned, adding water or medicinal extract for homogenating, and regulating the quality concentration of starch to be over 10 percent; (2) heating the homogenated fresh kudzuvine root for 10 to 20 minutes at the temperature of between 120 and 125 DEG C; (3) adding rhizopus, activated dried yeast, aroma-producing yeast and lactic acid bacteria into the sterilized mixture to ferment distilled wort, adding 10 percent lactic acid bacteria seed fermentation broth into the mixture when the alcohol content is detected to be between 6 and 8 percent, leading the fermentation temperature to be between 26 and 34 DEG C, adding edible salt into the mixture when the acidity of the fermentation broth is not changed, and stopping alcoholic fermentation; and (4) ageing the fermented acid solution for over 15 days at the temperature of between 12 and 20 DEG C, and filtering the solution to obtain the health-care vinegar. The health-care vinegar obtained by the brewing method can effectively inhibit CPK activity caused by myocardial ischemia and myocardial infarction, can reduce ET content (P is less than 0.05), has inhibition effect on LDH, can be used in preparation of medicament for preventing cardiovascular diseases, and has wide application prospect.