The invention relates to a screening and culture preparing method of a Russula.alutacea strain, belonging to the microbiological technical field. The fungus used in the invention is Russula.alutacea which was collected by China General Microbiological Culture Collection Center on December 15th, 2009 with the collection and register number of CGMCC.NO.3525. If applied to the propagation of Russula.alutacea, the good liquid and solid culture prepared from the strain has remarkable social and economic benefits and wide application prospects.