The invention discloses an enzyme-linked immunoassay kit for chlamys ferreri blood cells and a preparation method thereof. The kit comprises an enzyme labeled plate, confining solution, washing solution, enzyme labeled antibody, pNPP substrate developing solution, phosphate buffer solution, blood cell anticoagulant, chlamys ferreri blood cell monoclonal antibody and a blood cell standard sample. The preparation method for the kit comprises the following steps of: preparing the chlamys ferreri blood cell standard sample, preparing the blood cell monoclonal antibody, optimizing an optimal using proportion of the antigen and the antibody, and establishing a detection result assessment criteria. Detection results can assess the possibility that the chlamys ferreri to be detected is subjected to pathogenic infection or environmental stress and the like by judging the established assessment criteria so as to provide basis for monitoring the health condition of the chlamys ferreri.