The invention discloses a design method of a customizable network on chip based on a regular topology database, mainly solving the problem of rapid automatic generation of a topological structure with high performance. The design method comprises the steps of: firstly, inputting an application picture and an IP core configuration file according to the characteristics of the target application, and analyzing the performance requirements of the obtained application and an optimized target function; then analyzing the parameter functions of the obtained performance by utilizing a value function to generate the network topological structure, and initially establishing the topology by combining an AO* arithmetic and depth-first search; optimizing the network structure according to the communication requirement between an IP core and a core; and finally, designing a non-deadlock and an efficient route according to the characteristics of the generated layer topology, and establishing a communication network. The method has the advantages of high performance, strong expandability, small design complexity, capability of meeting the performance requirements of different targets and limiting to generate the topological structure, and automatic generation of the network on chip structure, and can be applied to the layout and the optimization of the domain of a multi-core chip.