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发明公开
CN101955526A 大黄鱼MSTN-1前肽和前肽抗体及制备方法 无效 - 驳回

大黄鱼MSTN-1前肽和前肽抗体及制备方法
摘要：
本发明公开了大黄鱼MSTN-1前肽和前肽抗体及制备方法，该大黄鱼MSTN-1前肽的氨基酸序列如序列表SEQIDNO.2所示，该前肽可以结合大黄鱼肌肉生长抑制素的功能区域，抑制肌肉生长抑制素的活性，促进大黄鱼的肌肉生长；大黄鱼MSTN-1前肽抗体可与该前肽结合，阻断该前肽与大黄鱼MSTN-1成熟肽结合，激活肌肉生长抑制素的活性，抑制大黄鱼肌肉的生长；大黄鱼MSTN-1前肽和前肽抗体的制备通过RNA提取、cDNA合成、目的片段制备、目的片段纯化、目的片段-pMD18-T-DH5α载体构建、大黄鱼MSTN-1前肽基因制备、pET-28a质粒的抽提和酶切、前肽基因-pET-28a-BL21载体构建、大黄鱼MSTN-1前肽制备及该前肽的免疫应答反应，可以得到大量的大黄鱼MSTN-1前肽和纯度较高的前肽抗体。
摘要（英）：

The invention discloses a Pseudosciaena crocea MSTN-1 (Myostatin) propeptide, a propeptide antibody and a preparation method thereof. The amino acid sequence of the Pseudosciaena crocea MSTN-1 propeptide is shown in a sequence table SEQIDNO.2. The propeptide can combine a functional region of a Pseudosciaena crocea MSTN for inhibiting the activity of the MSTN, and promoting muscle of Pseudosciaena crocea to grow. The Pseudosciaena crocea MSTN-1 propeptide antibody can combine the propeptide for inhibiting the propeptide combining with a Pseudosciaena crocea MSTN-1 mature peptide to activate the activity of the MSTN and inhibiting the muscle of Pseudosciaena crocea growing. The preparation method of the Pseudosciaena crocea MSTN-1 propeptide and the propeptide antibody comprises the steps of: RAN (ribonucleic acid) extraction, c DNA (c deoxyribose nucleic acid) synthesis, target fragment preparation, construction of target fragment-pMD18-T-DH alpha carrier, preparation of Pseudosciaena crocea MSTN-1 propeptide gene, extraction and enzyme digestion of pET-28a plasmid, construction of propeptide gene-pET-28a-BL21carrier, preparation of Pseudosciaena crocea MSTN-1 propeptide and propeptide immune response reaction. By the preparation method, a great amount of the Pseudosciaena crocea MSTN-1 propeptides and higher purity propeptide antibodies can be obtained.

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