The invention relates to a metal carrier Tenax coating solid-phase microextraction fiber relating to the field of trace analysis of organic matters contained in food, environment and biology samples. The metal carrier Tenax coating solid-phase microextraction fiber comprises a carrier and a coating, wherein the coating is positioned on the surface of the carrier; the carrier is a metal fiber with the length of 10-40 mm and the diameter of 0.1-0.5 mm; and the coating comprises a Tenax adsorbent and a high-temperature resistant epoxy resin layer or an organic silicon resin layer. The metal carrier Tenax coating solid-phase microextraction fiber has the advantages of higher mechanical strength, good heat stability of the coating and solvent washing resistance, and is suitable for the processing of samples, i.e. soil, atmosphere, essences, flavors, indoor air, alcohol, phenol, amine aldehyde, ketone, halogenated aryl hydrocarbon, and the like.