The invention relates to an efficient cell nucleus targeted medicine vector protein capable of quickly transporting an antineoplastic medicine into the cell nucleus and effectively killing tumor cells and a gene coding the efficient cell nucleus targeted medicine vector protein, belonging to the technical field of biopharmacy. The efficient cell nucleus targeted medicine vector comprises at leastone cell nucleus positioning sequence, at lest one transmembrane sequence and at least one polyhistidine sequence. The efficient cell nucleus targeted medicine vector is not only capable of enhancingthe sensitivity of the tumor cells to the antineoplastic medicine coupled with the vector, but also transporting the antineoplastic medicine into the cell nucleus of the tumor cells in a targeted way, thereby suppressing synthesis of nucleic acids of the tumor cells. Besides, the efficient cell nucleus targeted medicine vector has double actions of fastness and targeting, and greatly reduces the dosage of the antineoplastic medicine; and thus, the toxic and side effects of the antineoplastic medicine are reduced.