The invention discloses a remote temperature measuring method of magnetic nano-particles based on paramagnetic property. The method comprises the following steps: applying different excitation magnetic fields to a region at which a magnetic nano sample is positioned for multiple times; establishing an equation set of the different excitation magnetic fields and the corresponding magnetic susceptibility according to the Langevin paramagnetic principle; and solving temperature and sample concentration information according to the equation set. The method can be used for more precisely and quickly detecting the temperature of objects, and is especially suitable for detecting the thermal motion at a biomolecule layer; and tests show that the measuring error is less than 0.56 K.