The invention provides low-conductivity super-long-acting organic base type anti-freezing cooling liquid, which comprises the following components in percentage by weight: 15 to 96 percent of anti-freezing agent, 0.05 to 1.5 percent of natural alkaloid, 0.5 to 2.5 percent of hetero-cycloalkane, 0.01 to 0.3 percent of azole component, and the balance of deionized water. In invention, since the natural alkaloid is matched with nonionic organic compounds, the prepared anti-freezing cooling liquid has the characteristics of low freezing point, high boiling point, no scale formation, corrosion protection, high heat efficiency and the like; in addition, the prepared anti-freezing cooling liquid also has ultra-low conductivity which can reach 5 mus/cm and is a few hundredths of the conductivity of a common water cooling medium because the anti-freezing cooling liquid does not contain the components of inorganic salts, organic carboxylic acid and the like. The anti-freezing cooling liquid with ultra-low conductivity can effectively slow up the corrosion on metal parts of a cooling system, and meet the requirement of special conditions such as the cooling of a wind generator set and the like on the insulation safety of the anti-freezing cooling liquid. The anti-freezing cooling liquid realizes environmental protection and ultra-long-acting service, and is particularly suitable for special equipment such as a wind generator set, an imperial sedan engine and the like.