The invention discloses acid-resistant yeast and a method for producing an L-lactic acid recombinant strain through metabolic engineering construction, which belong to the technical field of genetic engineering. In the invention, the yeast which can grow under the condition that the pH value is 2.5 and is free from L-lactic acid is obtained by screening and separating and is identified as candidamagnoliae C4; the yeast is preserved in the China Center for Type Culture Collection, with the preservation number of CCTCCNO:M2010362; a coding gene (ldhA) of lactate dehydrogenase from rhizopus oryzae As3.819 is inserted into a yeast shuttle vector containing a G418 resistance gene to construct a recombinant plasmid, i.e., pYX212-kanMX-ldhA; and the recombinant plasmid is electrically converted into the candidamagnoliaeC4 to obtain a recombinant strain candidamagnoliae C42 with the capability of producing L-lactic acid, wherein the L-lactic acid yield of the recombinant strain candidamagnoliae C42 reaches 42g/L. The candidamagnoliae C42 constructed by the invention has the remarkable advantage of resisting lactic acid; and the consumption of CaCO3 as a neutralizing agent in the production can be greatly reduced, thus the pollution to the environment is reduced.