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发明公开
CN102206651A 一种大豆胞囊线虫抗性基因及其应用 失效 - 权利终止

一种大豆胞囊线虫抗性基因及其应用
摘要：
本发明公开了一种大豆胞囊线虫抗性基因及其应用。本发明提供的cDNA片段(SRC-J-6)，来源于大豆(Glycine max(L.)Merrill.)品种L-10，其核苷酸序列如以下1)-4)任一所示，1)其核苷酸序列如SEQ ID NO.1所示；2)在严格条件下与SEQ ID NO.1限定的核苷酸序列能够杂交且能够表达的DNA分子；3)与1)中所述核苷酸序列具有90％以上同源性，且能够表达的mRNA分子；4)与1)中所述核苷酸序列具有90％以上同源性，且能够表达的DNA分子。将本发明公开的基因转化植物组织后，能显著提高转基因大豆对胞囊线虫的抗性。本发明公开的大豆胞囊线虫抗性基因具有重要意义，不仅能有效指导常规育种，而且还可以为大豆的转基因育种事业提供优良的基因资源，在大豆生产中具有广阔的应用前景。
摘要（英）：

The invention relates to a soybean cyst nematode resistance gene and application thereof. The cDNA (complementary deoxyribonucleic acid) fragment (SRC-J-6) provided by the invention is derived from soybean (Glycine max (L.) Merrill.) variety L-10, and has a nucleotide sequence shown in 1) a nucleotide sequence shown in SEQ ID NO. 1; 2) a DNA (deoxyribonucleic acid) molecule capable of hybridizingwith the nucleotide sequence shown in SEQ ID NO. 1 and being expressed under strict conditions; 3) an mRNA (messenger ribonucleic acid) molecule, which has homology of more than 90% with the nucleotide sequence shown in 1) and can be expressed; or 4) a DNA (deoxyribonucleic acid) molecule, which has homology of more than 90% with the nucleotide sequence shown in 1) and can be expressed. After theplant tissues are transformed by the gene disclosed by the invention, the resistance to cyst nematode of the transgenic soybean is significantly improved. The soybean cyst nematode resistance gene provided by the invention has important significance, not only can effectively guide conventional breeding, can also provide excellent genetic resources for the transgenic breeding of soybean and has broad application prospects in the soybean production.

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