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发明公开
CN102433312A 一种D-3-磷酸甘油酸脱氢酶及其编码基因及构建方法 无效 - 驳回

一种D-3-磷酸甘油酸脱氢酶及其编码基因及构建方法
摘要：
本发明涉及一种D-3-磷酸甘油酸脱氢酶，其氨基酸序列见序列1，其L-丝氨酸的抑制常数Ki＞250mM，以及一种D-3-磷酸甘油酸脱氢酶的编码基因，基因序列见序列2。本发明提供的PGDH的氨基酸序列与大肠杆菌野生型PGDH的氨基酸序列(序列3)的不同之处在于第344位氨基酸不是组氨酸而是丙氨酸且第346位氨基酸不是天冬酰胺而是丙氨酸，突变产生的PGDH不受L-丝氨酸的抑制，且本发明的PGDH变体的酶活性与野生型相比未发生改变。
摘要（英）：

The invention relates to D-3-phosphoglycerate-dehydrogenase and a coding gene thereof. The amino acid sequence of the D-3-phosphoglycerate-dehydrogenase is shown as a sequence 1; the inhibition constant Ki of L-serine of the D-3-phosphoglycerate-dehydrogenase is greater than 250mM; and the sequence of the coding gene is shown as a sequence 2. The difference between the amino acid sequence of the D-3-phosphoglycerate-dehydrogenase (PGDH) provided by the invention and the amino acid sequence (sequence 3) of the escherichia coli wild type PGDH lies in that amino acid at 344th site is not histidine but lactamine and amino acid at 346th site is not asparagines but lactamine and the PGDH generated by mutation is not inhibited by the L-serine. In addition, the enzyme activity of a PGDH variant disclosed by the invention has no change compared with the wild type PGDH variant.

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