The invention discloses a preparation method of a microecological zymophyte agent for Szechuan pickled bamboo shoots. The preparation method comprises the steps of: taking 10L of bamboo shoot pickling solution supplied by Chengdu Xinfan food Co., Ltd. to server as a promoter of the microecological zymophyte agent, feeding the bamboo shoot pickling solution into a 1000L fermentation tank for cultivation, and carrying out anaerobic fermentation for 48h at the initial pH value being 5.8 and the fermentation temperature being 27 DEG C; filtering fermentation broth through a 100-mesh filter, adding porous starch, maltodextrin and polyvinylpyrrolidone which respectively account for 10%, 15% and 0.15% relative to the weight of filtrate into the filtrate, carrying out spray drying, adding tricalcium phosphate accounting for 7% relative to the weight of the filtrate to serve as a filling agent, and detecting bacterial count by using a plate count method to obtain powdery microecological zymophyte agent, with the viable count being about 1010-1011CFU/g, for the Szechuan pickled bamboo shoots. The Szechuan pickled bamboo shoots fermented by using the microecological zymophyte agent are bright in color, glossy, strong in acid aroma and pure and natural in fermentation aroma and are not obviously different from the traditional pickled bamboo shoots in flavor.