The invention relates to a cleaning method in the immunoassay process, which utilizes an ultrasonic cleaning instrument and includes steps of placing test strips or microplates to be cleaned in the immunoassay process into a container containing cleaning solution firstly, keeping cleaned portions of the test strips or microplates immersed by the cleaning solution, placing the container into the ultrasonic cleaning instrument, and then starting the ultrasonic cleaning instrument for cleaning. The ultrasonic cleaning instrument serving as the cleaning equipment has low requirements, and frequency and power of the ultrasonic cleaning instrument can be regulated to control the cleaning process, so that the cleaning process can be completed by simple operations in a labor-saving and time-saving manner while cleaning effect is quite fine.