The invention relates to a fluorescence nanometer probe, comprising an inner core formed by polyglycolide lactide, a middle layer formed by phospholipid surrounding on the surface of the inner core and a shell formed by distearoyl phosphatidyl ethanolamine-polyethylene glycol which contains amino or carboxyl and partially penetrates through the middle layer, wherein indocyanine green is dispersed in the inner core. According to the invention, a core-shell structure is formed to ensure that the indocyanine green is wrapped in the polyglycolide lactide, therefore, the indocyanine green is effectively avoided from being aggregated and decomposed and the stability is increased; and the wrapped indocyanine green has a near-infrared fluorescence characteristic to ensure that the background fluorescence penetrating tissues is small and then can be relatively accurately applied to bioluminescence labeling.