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发明公开
CN102533655A 高效表达人源重组蛋白GLP1/Fc的CHO-S细胞株及其建立方法 无效 - 驳回

高效表达人源重组蛋白GLP1/Fc的CHO-S细胞株及其建立方法
摘要：
本发明提供了一种高效表达人源重组蛋白GLP1/Fc的细胞株CHO-S，通过将带有人源GLP1/Fc基因的质粒转染至CHO-S细胞，经筛选后，获得稳定、高效表达人源重组蛋白GLP1/Fc的CHO-S(GLP1/Fc)单克隆细胞株。本发明获得的CHO-S(GLP1/Fc)单克隆细胞株分泌产生GLP1/Fc融合蛋白，GLP1/Fc融合蛋白直接作用于GLP-1受体，促进胰脏β-细胞再生，分泌胰岛素和葡萄糖耐受性，促进β-细胞的生长，增加β-细胞的数量，能使II型糖尿病人的β-细胞功能恢复到正常水平。
摘要（英）：

The invention provides a cell strain CHO-S for efficient expression type human recombinant protein GLP1/Fc. A plasmid with a human GLP1/Fc gene is transfected into a CHO-S cell, and a CHO-S (GLP1/Fc) monoclonal cell strain for stable and efficient expression of the human recombinant protein GLP1/Fc can be obtained after screening. GLP1/Fc fusion protein can be produced via secretion of the CHO-S (GLP1/Fc) monoclonal cell strain and can directly act on a GLP-1 receptor to promote pancreatic beta-cells, insulin and glucose tolerance can be achieved via secretion, the growth of beta-cells can be promoted, the number of beta-cells can be increased, and the normal levels of the functions of the beta-cells of patients suffering from II type diabetes can be restored.

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