The invention discloses a tabletting method of a plum blossom shoot tip chromosome. The method comprises the following steps: successively carrying out a Kano stationary liquid fixation, front low permeability treatment, cellulase and pectinase enzymolysis and back low permeability treatment on a part of 0.5-1.0cm at the innermost side of a top growth point of an annual branch of plum blossom or the top growth point germinated newly after pruning; and carrying out flame dry-method tabletting on the treated top growth point. According to the invention, tabletting is carried by adopting a flame method, and pretreatment, dissociation and staining and tabletting processes are omitted to directly carry out fixation, thereby improving tabletting efficiency and elongating the length of the chromosome. In addition, more importantly, the obtained tablet can be used for a fluorescence in-situ hybridization experiment, and simultaneously, the problem that sampling is limited by early spring is solved.