The invention relates to a synthesis method of natural alpha-tocopherol acetate, which comprises the following steps: preparing immobilized lipase, modifying the immobilized lipase, carrying out catalytic reaction on the immobilized lipase, filtering and purifying. Natural alpha-tocopherol and an acetyl donor are subjected to esterification or ester exchange reaction under the catalytic action of the modified immobilized lipase, and the reaction product is filtered and subjected to reduced pressure distillation and molecular distillation. The catalyst has the advantages of low consumption and no equipment corrosion, and can be easily recycled; in the reaction process, the invention has the advantages of fewer introduced chemical reagents, no side reaction and high reactant conversion rate (up to more than 90%), can simplify the separation, can not generate abundant wastewater, and does not need to recycle abundant extraction solvent, thereby being environment-friendly and energy-saving; and thus, the invention satisfies the requirements for large-scale continuous industrial production.