The invention provides an indirect ELISA (enzyme linked immunosorbent assay) detection method for an escherichia coli OmpT (Outer-membrane protease T) antibody, and a detection antigen on an ELISA plate in the ELISA is OmpT recombinant protein. The detection method comprises the following steps that after being diluted according to the ratio of 1:640, the serum to be detected is incubated with the OmpT recombinant protein on the ELISA plate at the temperature of 37 DEG C for 1.5 h, and the plate is washed three times; rabbit anti-bovine IgG marked by horse radish peroxidase is added to be incubated with the serum to be detected at the temperature of 37 DEG C for 1.5 h; a TMB (tetramethylbenzidine) substrate is added for developing for 10 min; and OD450 is detected after the reaction is ended, the antibody is positive if the OD450 is larger than or equal to 0.335, and the antibody is negative if the OD450 is smaller than 0.335. The recombinant protein prepared from the symbolic gene OmpT of the escherichia coli is used as the detection antigen for ELISA, the recombinant antigen can be produced on a large scale in a standardizing mode, has strong specificity, and is suitable for the antibody detection after the infection of all serotype bacteria of the escherichia coli.