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发明公开
CN102766643A 编码5-烯醇式丙酮酰莽草酸-3-磷酸合酶的基因aroA及其应用 无效 - 撤回

编码5-烯醇式丙酮酰莽草酸-3-磷酸合酶的基因aroA及其应用
摘要：
本发明公开了一种编码5-烯醇式丙酮酰莽草酸-3-磷酸合酶的基因aroAS001及其应用，该基因的碱基序列如SEQ ID NO.3所示；该基因可用于提高农作物的草甘膦抗性，具体的是将基因aroAS001、含有基因aroAS001的表达载体或克隆载体，或含有上述的表达载体或克隆载体的菌株转化入农作物中。本发明的基因是一种优良的草甘膦抗性基因，转入该基因的aroA基因缺陷型菌株能在含400mM以下草甘膦的培养基中生长，与目前已报道的其它物种EPSP合成酶基因相比，表现出优良的草甘膦抗性，取得了意料不到的有益效果。
摘要（英）：

The invention discloses a gene aroAS001 for encoding 5-enolpyruvyl-shikimate-3-phosphate synthase, and an application of the gene, wherein the base sequence of the gene is represented by SEQ ID No.3; the gene is suitable for enhancing glyphosate resistance of crops; specifically, gene aroAS001, and an expression vector or a cloning vector containing gene aroAS001, or strains containing the expression vector or cloning vector are transferred in the crops. The gene provided by the invention is an excellent glyphosate resistant gene, the aroA gene defect type strains transferred with the gene can grow in a culture medium containing lower than 400mM glyphosate; compared with other known EPSP (enolpyruvyl-shikimate-3-phosphate) synthase genes, the gene provided by the invention has significant glyphosate resistance and brings about unexpected beneficial effects.

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