The invention discloses a method for separating and purifying all-trans lutein esters powder prepared by Tagetes erecta granules, comprising the steps of mixing the Tagetes erecta granules and nonpolar solvent to perform continuously countercurrent supersonic extraction to obtain extract liquid, concentrating the extract liquid to obtain crude extract liquid extracts, dissolving the crude extract liquid extracts by solvent and using alumina column to perform column chromatography to obtain the high-purity lutein esters products. According to the invention, the continuously countercurrent supersonic extraction is adopted to extract lutein esters, and the method has large batch processing capacity, less solvent using amount and high recovery rate, can reduce labor and power expenses, solves the technical problem that the lutein esters are directly purified from crude Tagetes erecta extracts by adopting the alumina column to perform chromatography of the lutein esters, and can prepare the all-trans lutein esters products with content more than 95% (HPLC: High Performance Liquid Chromatography), wherein the total carotenoid yield is more than 80 percent.