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发明公开
CN102866219A 一种马铃薯中α-茄碱提取与检测的方法 失效 - 权利终止

一种马铃薯中α-茄碱提取与检测的方法
摘要：
本发明公开了一种马铃薯中α-茄碱提取及检测的方法，其步骤包括：(1)α-茄碱提取：取新鲜马铃薯肉，用组织匀浆机粉碎成匀浆，称取样品置于瓶中，加入提取液，密封后，超声提取，将提取液过膜备用；所述的提取液为体积比=1:1的质量浓度1%甲酸-水/甲醇、体积比=1:1的质量浓度1%盐酸-水/甲醇、质量浓度0.5%甲酸-水或质量浓度0.5%盐酸-水；所述的超声提取在超声频率35kHz下提取时间为5～15min。(2)液质联用检测α-茄碱：以α-茄碱m/z[M+H]+=868.78为目标离子，用选择离子监测模式定量，将标准对照液进样检测，以浓度（X，ppb）为横坐标，以峰面积（Y）为纵坐标，绘制标准曲线，得到回归方程，根据回归方程计算α-茄碱的含量。该方法能简单、快捷、准确地检测出马铃薯中α-茄碱。
摘要（英）：

The invention discloses a method for extracting and detecting alpha-solanine in a potato. The method comprises the following steps of: (1) extracting the alpha-solanine: preparing fresh potato pulp, smashing the potato pulp into homogenate by a tissue homogenate machine, weighing a sample and putting the sample into a bottle, adding extracting solution, carrying out ultrasonic extraction after sealing the bottle, and carrying out film pasting on the extracting solution to be standby; the extracting liquid is methanoic acid-water/methanol in which the mass concentration of the methanoic acid is 1% and the volume ratio of methanoic acid and water/ methanol is 1:1, hydrochloric acid-water/methanol in which the mass concentration of the hydrochloric acid is 1% and the volume ratio of hydrochloric acid and water/ methanol is 1:1, methanoic acid-water in which the mass concentration of the methanoic acid is 0.5%, or hydrochloric acid/water in which the mass concentration of the hydrochloric acid is 0.5%; and the extraction time of the ultrasonic extraction under an ultrasonic frequency of 35 kHz is 5-15 min; and (2) carrying out liquid chromatography-mass spectrometry detection on the alpha-solanine: taking alpha-solanine m/z[M+H]+=868.78 as a target ion, carrying out quantifying by a selective ion monitoring mode, detecting a standard reference liquid, drawing a standard curve by taking concentration (X, ppb) as a horizontal coordinate and peak area (Y) as a vertical coordinate, and thus obtaining a regression equation, and calculating the content of the alpha-solanine according to the regression equation. According to the method for extracting and detecting the alpha-solanine in the potato, the alpha-solanine in the potato can be simply, quickly and precisely detected.

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