The invention provides a high-activity strain capable of realizing malolactic conversion. The high-activity strain is Lactobacillus plantarum subsp. Plantarum RF17 which is preserved in China Center for Type Culture Collection in Wuhan University on Mar.16, 2012, with the preservation number of CCTCC NO: M 2012085. The high-activity strain capable of realizing malolactic conversion, provided by the invention has the advantages that by utilizing the strain, namely the Lactobacillus plantarum subsp. Plantarum RF17, the content of malic acid in fruit wine and fruit juice can be reduced more efficiently, content of lactic acid, volatile ester and the like in the fruit wine and the fruit juice can be increased, so that the fruit wine and the fruit juice is more gentle and coordinated in mouthfeel, and is sweet-scented in flavor, a solid foundation is laid to improve quality of the fruit wine and the fruit juice, and therefore a novel strain source is provided for the research on the biological acid-degradation and lactic acid fermentation of the fruit wine.