The invention relates to a natural medicine, and in particular relates to a novel isoflavanone isoflavanone compound in wild peanuts as well as a preparation method and application of the compound. The novel isoflavanone isoflavanone compound provided by the invention has a structural formula shown in a formula (I). The preparation method comprises the following steps of: a, crushing; b, refluxing and extracting; c, concentrating; d, extracting; and e, separating. The novel isoflavanone isoflavanone compound shown in the formula (I) has a remarkable inhibition function to a human cervical carcinoma, human chronic myeloid leukemia or human acute myelogenous leukemia cell strain, has a better anti-cancer activity, and can be used as an anti-cancer active component or lead compound. The novel isoflavanone isoflavanone compound can be a natural organic compound, is wide in raw material source, is simple in preparation process, is high in purity, is easily realized in subsequent industrialized production, is used for preparing a medicament for preventing or treating tumors, and is used for clinically treating human cervical carcinoma, human chronic myeloid leukemia or human acute myelogenous leukemia.