The invention discloses a Radix notoginseng culture medium prepared by using zeolite, perlite and organic fertilizers. The volume ratio of the zeolite to the corn stalk fermented organic fertilizers to the perlite is specifically 6:2:2, 6:3:1 or 5:3:2. The zeolite is natural zeolite. The collected natural zeolite is crushed and then is screened through a 10-mesh sieve to obtain screened zeolite which is used as a medium ingredient for future use; and the processed zeolite, the organic fertilizers and the perlite are fully mixed according to the proportions and the obtained mixture is sterilized at high temperature to obtain the seedling culture medium. On one hand, the prepared culture medium does not carry pathogenic bacteria and diseases of Radix notoginseng can be well relieved; and on the other hand, the zeolite used for preparing the culture medium has the characteristics of high trace element content, good air permeability and strong water and fertilizer conserving capacity, and the corn stalk fermented organic fertilizers have rich nutrients and appropriate physical structures.