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发明公开
CN103060345A 乌贼精氨酸激酶基因及其原核表达载体和工程菌 无效 - 撤回

乌贼精氨酸激酶基因及其原核表达载体和工程菌
摘要：
本发明公开了一种乌贼精氨酸激酶基因及其原核表达载体pET30a-AK，具体涉及到一个与细胞内能量运转、肌肉收缩、ATP再生有直接关系的重要激酶——精氨酸激酶(Arginine Kinase，AK)及其原核表达载体。本发明是利用RACE PCR技术从乌贼肝脏中克隆了AK基因的全长cDNA，然后将其蛋白编码序列克隆到原核表达载体pET30a-AK上，并在大肠杆菌表达菌株BL21中进行了表达与纯化。分离得到的重组蛋白，不仅可作为抗原用于AK抗体的制备，并对研究无脊椎动物体内的能量代谢调节规律具有重大价值。
摘要（英）：

The invention discloses a cuttlefish arginine kinase gene and a prokaryotic expression vector pET 30a-arginine kinase (AK) thereof, and particularly relates to an important kinase--arginine kinase (AK) and a prokaryotic expression vector thereof. The arginine kinase (AK) is directly related to intracellular energy operation, muscle contraction and adenosine triphosphate (ATP) regeneration. Total-length complementary deoxyribonucleic acid (cDNA) of the AK gene is cloned from a liver of a cuttlefish by means of RACRPER technology, then a protein coding sequence of the AK gene is cloned to the prokaryotic expression vector pET 30a-AK, and expression and purification are carried out in an escherichia coli expression strain BL21. Recombinant proteins are obtained through separation, not only can be used as antigens for preparation of AK antibodies, but also have great value in research of an invertebrate intracorporal energy metabolism regulating law.

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