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发明公开
CN103060354A 一种组合式可激发型荧光蛋白及其应用 有权

一种组合式可激发型荧光蛋白及其应用
摘要：
本发明公开了一种组合式可激发型荧光蛋白及其应用。本发明提供的用于检测待测蛋白相互作用的载体组，为如下1)或2)：1)由含有蛋白A1的编码基因的重组载体A1和含有蛋白B1的编码基因重组载体B1组成；2)由含有蛋白A2的编码基因的重组载体A2和含有蛋白B2的编码基因重组载体B1组成。实验证明，本发明提供了一套新型组合式可激发型荧光蛋白示踪系统、在单分子水平监控蛋白质的相互作用，利用NDC80复合物融合蛋白对该系统进行了检测。该系统将成为功能蛋白组学时代生物光子学领域的前沿技术，其应用前景十分可观。
摘要（英）：

The invention discloses a combined excitable fluorescent protein and application thereof. The vector group for detecting interaction of protein to be detected is 1) or 2): 1) composed of a coding gene recombinant vector A1 containing protein A1 and a coding gene recombinant vector B1 containing protein B1; and 2) composed of a coding gene recombinant vector A2 containing protein A2 and a coding gene recombinant vector B2 containing protein B22. The experiment proves that the invention provides a novel combined excitable fluorescent protein tracing system and interaction for monitoring protein on the monomolecular level; and an NDC80 composite fusion protein is utilized to detect the system. The system can become a leading edge technology in the field of biological photonics in the age of functional proteomics, and has considerable application prospects.

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