The invention relates to a layered sampling testing device for a capillary water zone. n unit columns are connected is series and stacked, so that the device is formed; a separation net of each unit column is embedded into a net embedding plate; a sealing groove is a shallow-groove circular ring with the width of about 20mm and the depth of 1mm, and the shallow-groove circular ring is polished along the inner edge of the net embedding plate to the outer edge, and the diameter and the depth of the circular ring are approximately equal to the diameter and the thickness of the separation net; the lower surface of a lower flange, the edges of the sealing groove and the upper surface of the net embedding plate are painted with organic glass cement uniformly, so that a lower flange disk and the net embedding plate are attached together, and the separation net and an organic glass column pipe are fixed closely further; sizes of an upper flange and the lower flange of each unit column are the same with those of the inner diameter and the outer diameter of the net embedding plate, and a lower flange disk of a previous unit column can be stacked on an upper flange disk of a unit column of a next layer in a seamless manner; rib plates are arranged in position of joints of the upper flange disk, the lower flange disk and the organic glass column pipe; and the unit columns are connected through screws in flange screw holes, so that the unit columns are sealed, connected in series and stacked. The device is simple in structure, low in manufacture and maintenance costs, convenient in operation and high in applicability, and can be popularized and applied widely in fields such as civil engineering, geotechnical engineering and the like for physical simulation researches on various rock and earth masses under the action of capillary water.