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CN103215226A Lmx1a介导的与神经干细胞共培养诱导rASCs为DA能神经元的方法 无效 - 撤回

Lmx1a介导的与神经干细胞共培养诱导rASCs为DA能神经元的方法
摘要：
Lmx1a介导的与神经干细胞共培养诱导rASCs为DA能神经元的方法，属于诱导干细胞替代缺损的神经元细胞治疗帕金森氏病的方法。本发明步骤有构建Lmx1a基因克隆及重组质粒，构建与包装重组腺病毒Ad-Lmx1a，将感染Ad-Lmx1a的rASCs与NSCs共培养分化为DA能神经元；以及rASCs和NSCs传代细胞的培养等。本发明诱导的神经元样细胞能分化出表达多巴胺能神经元细胞蛋白标志物，且诱导后的细胞可传导电信号或分泌神经递质，证明诱导细胞为神经元细胞。
摘要（英）：

A method for co-culturing with nerve stem cells to inducing rASCs to be DA Neurons by Lmx1a mediation, belongs to a method for treating Parkinson's disease by inducing stem cells to replacing defective Neuron cells. The method of the invention comprises steps of: building Lmx1a gene clone, recombining plasmid, building, packaging and recombining adenovirus Ad-Lmx1a, and co-culturing rASCs and NSCs which are infected by Ad-Lmx1a to differentiating them to be DA Neurons; and culturing rASCs and NSCs passage cells. Protein marker for expressing dopaminergic neuron cells can be differentiated from inducted neuron-like cells in the invention, and the inducted cells can transmit electric signal or secrete neurotransmitters, to prove that the inducted cells are the Neuron cells.

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