The invention belongs to the technical field of biological engineering, and relates to the construction, strain, and application of a microbial fuel cell electricity-producing genetically engineered bacteria. With a molecular biology method, a common key gene nadE in Escherichia coli NAD<> de-novo synthesis pathway and anaplerotic pathway is heterologously expressed in pseudomonas aeruginosa PAO1, such that the high-electricity-production genetically engineered strain pseudomonas aeruginosa PA-nadE is obtained, and can be applied in the development of the field of microbial fuel cell. Also, good basis is provided for the genetic engineering researches of electricity-producing pseudomonas aeruginosa.