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发明公开
CN103275981A 丝状真菌蛋白分泌压力反馈调控元件与抗反馈抑制的启动子、质粒及制备方法和转化细胞 有权

丝状真菌蛋白分泌压力反馈调控元件与抗反馈抑制的启动子、质粒及制备方法和转化细胞
摘要：
本发明公开一种丝状真菌蛋白分泌压力反馈调控元件与抗反馈抑制的启动子、质粒及制备方法和转化细胞，该调控元件为丝状真菌启动子-378bp～-291bp区域的碱基序列。该调控元件与蛋白分泌压力反馈相关。抗反馈抑制的启动子为丝状真菌启动子上至少缺失或者替换掉-378bp～-291bp区域的碱基所得的碱基序列、并且具有启动子活性。本发明的启动子可以在丝状真菌，特别是在米曲霉中蛋白能够高效表达。
摘要（英）：

The invention discloses a promoter for a filamentous fungal protein secretion pressure feedback regulation element and feedback inhibition resistance, a plasmid, a preparation method and a transformed cell. The regulation element is a base sequence of the area from (-378bp)-(-291bp) of a filamentous fungal promoter. The regulation element is related to the protein secretion pressure feedback. The promoter against feedback inhibition is a base sequence obtained by at least losing or replacing the base of the area from (-378bp)-(-291bp) on the filamentous fungal promoter, and has promoter activity. The promoter disclosed by the invention can be efficiently expressed in filamentous fungi and particularly in the protein of aspergillus oryzae.

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