The invention discloses a method for killing Clonorchis sinensis metacercaria in sashimi. Sashimi immersed in an ice-water mixture undergoes ultrasonic wave treatment having a work frequency of 20-24KHz under an ultrasonic power of 10-80W for 28s-5min in a 2s implementation and 2s intermittence manner. The method has the advantages of simple technology, short time consumption, effective prevention of the Clonorchis sinensis metacercaria from infecting human bodies, and basically no influences of the quality of the fish flesh.