The invention discloses a positioning method of overall puncture module equidistance densely-arrayed micro holes. The method comprises the steps that a mini-sized CCD camera is demarcated to eliminate image distortion; a steel needle array of overall puncture is determined; binaryzation is conducted on an image and the image is signed, point-by-point traversal is conducted, then the distances between the center point of a cross cursor and four end points are controlled to be equal, and the fact that poisoning holes and needle distribution basic holes are coaxial; the relative position relationship between the needle falling holes and the needle distribution basic holes is calculated and obtained according to the relative position relationship between the needle falling holes and the positioning holes; a base plate is controlled to move transversely and longitudinally according to the steel needle array of the overall puncture, and therefore the positioning of the overall puncture module equidistance densely-arrayed micro holes is achieved. The positioning method of the overall puncture module equidistance densely-arrayed micro holes is used in the carbon fiber three dimensional fabric overall puncture technology, and the needle distribution basic holes are defined to the mini-sized holes on the upper left corner of a puncture template. The positioning method of the overall puncture module equidistance densely-arrayed micro holes provides necessary center position information of the needle distribution basic hole for a steel needle array distributing mechanism on the basis of the image and a cross-shaped laser, and puncture steel needles can be distributed conveniently.