The invention discloses an immunofluorescent chromatographic test strip and a detection method for HIT (Heparin-induced thrombocytopenia) antibodies. The test strip comprises a sample pad, a reaction membrane and an adsorption pad which are overlapped with one another on a liner plate, wherein the two ends of the sample pad are respectively provided with a first sample adding point and a second sample adding point, and the reaction membrane is fixedly provided with an internal quality control belt and at least one antibody detection belt. The detection method comprises the following steps of: adding a sample to be detected into the sample pad, and then adding a fluorescently labeled secondary antibody to be detected by a fluorescence detection instrument. Through the mode, according to the immunofluorescent chromatographic test strip and the detection method for the HIT antibodies, provided by the invention, the detection process is simple, efficient, accurate, cheap and practical, and the semi-quantitative or quantitative detection can be realized. The invention provides a relativley reliable and practical method for diagnosis of clinical heparin-induced thrombocytopenia and related complications in China.