The invention discloses a method for dual fluorescence quantitative PCR detection of grass carp reovirus types I and II. The method can realize that the grass carp reovirus types I and II can be simultaneously detected in one to-be-detected sample, and the load levels of the two reovirus types can be quantified. The invention further provides a kit for dual fluorescence quantitative PCR detection of the grass carp reovirus types I and II. According to the detection method and the kit which are provided by the invention, the operation is simple and convenient, the specificity is strong, the detection sensitivity and accuracy rate are high and reliable, and a wide application prospect is achieved.