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发明公开
CN103497972A 一种带有荧光素酶基因的JEV感染性克隆及构建方法和应用 失效 - 权利终止

一种带有荧光素酶基因的JEV感染性克隆及构建方法和应用
摘要：
本发明公开了一种带有荧光素酶基因的JEV感染性克隆及构建方法和应用。一种带有荧光素酶基因的JEV感染性克隆，其制备步骤是：（1）JEV病毒SA14株基因序列的分段合成；（2）JEV病毒感染性克隆的组装与构建；（3）带有荧光素酶基因的JEV感染性克隆的构建。本发明通过免疫荧光、Rluc活性的检测、病毒噬斑、药物抑制、活体动物成像及疫苗评价等实验证明：本发明所构建的带有荧光素酶基因的JEV感染性克隆可以拯救出具有与JEV病毒生长趋势相同的Rluc-JEV报告病毒，并且在动物模型、病毒复制与致病机理、药物筛选和药物作用机制、活体动物成像及疫苗评价等方面具有广泛的应用价值。
摘要（英）：

The invention discloses a Japanese encephalitis virus (JEV) infectious clone with a luciferase gene, and a building method and application thereof. The JEV infectious clone with the luciferase gene is prepared by the following steps: (1) sectional synthesis of a JEV SA14 strain gene sequence; (2) assembling and building of the JEV infectious clone; (3) building of the JEV infectious clone with the luciferase gene. It is proved that a Rluc-JEV report virus with the same growth tendency as the JEV virus can be saved by the JEV infectious clone with the luciferase gene built by the method, and the JEV infectious clone has wide application value in the aspects of an animal model, virus replication and pathogenesis, drug screening and drug action mechanism, live animal imaging and vaccine evaluation and the like through the experiments of immunofluorescence, Rluc activity detection, virus bacteriophage plaque, drug inhibition, live animal imaging, vaccine evaluation and the like.

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