The invention discloses a cultivation method of rice capable of resisting black-streaked dwarf. The method comprises the following steps: constructing a RNAi plant expression vector containing the sectional fragments of a rice black-streaked dwarf virogene group S7; guiding target fragments into the embryonic calluses of the rice by utilizing an agrobacterium-mediated transgenosis method; performing hygromycin resistance screening and differentiation so as to obtain a regenerated transgenic plant; and analyzing the virogene expressions of the transgenic plant subjected to a virus access and virus transmission test, thereby showing the fact that the expressions of the virogenes in the transgenic rice plant are obviously decreased. The transgenic rice plant capable of resisting the black-streaked dwarf can be cultivated by utilizing an RNAi technology, so that the problem of lacking resources for resisting the rice black-streaked dwarf is radically solved; and the resistance of the rice to the black-streaked dwarf is obviously enhanced. As a result, the economic benefit is improved.