The invention relates to applications of an EZTI gene in the technical field of bioengineering and a method of causing rice male sterility by recovering EAT1 gene deletion. The coding of the EAT1 gene is amino acids shown as SEQ ID NO.3. The applications relate to that rice male sterile strains can be obtained by gene mutation or suppression expression and the EZT1 gene can be used for producing seeds. The invention also relates to the method causing the rice male sterility by recovering the EAT1 gene deletion. A mutant can be recovered to a wild-type phenotype by EAT1 gene amplification through a primer, and by transformation of a mutant plant through a genetic transformation manner. A rice mutant obtained by the method has no abnormity in a vegetative growth period, but a homozygote plant is entirely sterile. The applications and the method, if are applied for crossbreeding, can avoid the work of castration in the female parent, largely improve the production efficiency and reduce the labor cost, and have important applications in agriculture production.