The invention provides a hepatitis B surface antibody measurement kit which comprises a reagent M, a reagent R and a calibration material, wherein the component in the reagent M comprises streptavidin coated magnetic particles, wherein the streptavidin coated magnetic particles are treated by a diagnostic reagent preservative; the components in the reagent R include biotinylated HBsAg, acridinium ester-labeled HBsAg, a PB buffer solution and a diagnostic reagent preservative; a control group comprises a human hepatitis B surface antibody, new-born calf serum and a diagnostic reagent preservative; the biotins in the biotinylated HBsAg and streptavidin in the streptavidin coated magnetic particles form a biotin-avidin system; the biotin-avidin system is a separation system after immunoreaction of the kit measurement process, so that the magnetic particles have high universality. The hepatitis B surface antibody measurement kit provided in the invention is simple in preparation, convenient to use and easy to store. The detection method using the kit is based on a double-antigen chemiluminescence principle and is high in sensitivity, small in error, safe and rapid.