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发明公开
CN103642763A 一种7α，15α双羟化DHEAP450酶基因的克隆及分析 有权

一种7α，15α双羟化DHEAP450酶基因的克隆及分析
摘要：
本发明提供一种来自于Colletotrichum lini ST-1的新型真菌细胞色素P450酶CYP X完整的mRNA和DNA序列的克隆和分析方法，其核苷酸序列分别为SEQ ID NO:1和SEQ ID NO:2，相应的氨基酸序列命名为SEQ ID NO:3。该酶能高效转化DHEA为7α,15α-diOH-DHEA，该基因的获得为其异源表达及产业化生产奠定理论基础，有较大的应用潜力及经济价值，也为细胞色素P450酶的相关研究奠定了理论基础。
摘要（英）：

The invention provides a cloning and analysis method for complete mRNA and DNA sequences of a novel fungal cytochrome P450 enzyme CYP X from Colletotrichum lini ST-1. The nucleotide sequences are SEQ ID NO:1 and SEQ ID NO:2 respectively, and a corresponding amino acid sequence is named as SEQ ID NO:3. The enzyme can efficiently convert DHEA into 7alpha, 15alpha-diOH-DHEA. Obtaining of the gene lays the theoretical foundation for heterologous expression and industrial production. The gene has great application potential and economic value, and also lays the theoretical foundation for related research on cytochrome P450 enzyme.

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