The invention relates to an anti-explosion partition structure between two tunnels. A connecting channel is arranged between the two bi-directional tunnels parallel to each other, and a partition device convenient to open and capable of resisting explosion and isolating smoke is needed. Reinforcing bushings are arranged at the two ends of the connecting channel between the two tunnels and the inner end of a lining of the connecting channel, a reinforced concrete door frame wall is arranged at the inner ends of the reinforcing bushings and provided with a pre-buried steel door frame, and an anti-explosion door is installed on the pre-buried steel door frame. A reinforcing distributed steel hidden beam is transversely arranged above the reinforced concrete door frame wall, and a top cavity seal filled with reinforced concrete is arranged between the upper portion of the reinforcing distributed steel hidden beam and the reinforcing bushings. A small V-shaped mortar layer is arranged at the bottom of the reinforced concrete door frame wall. The anti-explosion partition structure is convenient to open, easy to construct, convenient to maintain, safe and reliable and can resist explosion and partition smoke between the two tunnels.