The invention discloses a method for preparing human plasma protein C concentrate, which comprise the following steps of: (1) gel adsorption: fully swelling gel, balancing by using equilibrium liquid, adding cryoprecipitate-reduced plasma with addition of 250-750 times (v/w) of the gel, carrying out stirring for adsorption, and carrying out supernatant removal, washing and elution to obtain eluent; (2) ultrafiltration and dialysis: carrying out ultrafiltration on the eluent to obtain a concentrated solution, and carrying out dialysis until the electric conductivity is lower than 10mS/cm; (3) S/D inactivation; (4) heparin affinity chromatography: balancing a heparin affinity chromatography column by using a buffer solution, carrying out loading, washing by using a buffer solution with sodium chloride concentration of 0.01-0.1M, and eluting by using a buffer solution with sodium chloride concentration of 0.1-0.18M to obtain eluent; and (5) carrying out ultrafiltration, dialysis, sterile subpackage, freeze-drying and dry heat inactivation of virus. The preparation is simple, low in cost and high in yield; and the prepared product is high in purity, strong in activity and favorable in industrial application prospect.