The invention discloses pseudozyma fusiformata for inhibiting diseases after fresh fruit collection and application thereof, belonging to the technical field of microorganism application. The pseudozyma fusiformata SF6 disclosed by the invention has the preservation number of CGMCC No.8577, has inhibiting effects on monilinia after peach collection and botrytis cinerea after tomato collection, has inhibiting effects on the brown rot after peach collection and the grey mould after tomato collection, and has the most obvious inhibiting effect on the monilia, and the diameter of the inhibition zone is more than 15mm. The pseudozyma fusiformata has stable, efficient and broad-spectrum antimicrobial properties, and can be applied to prevention and treatment of the germs or diseases.