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发明公开
CN104017807A 猪骨骼肌特异性表达基因MyoG启动子的克隆及应用 无效 - 撤回

猪骨骼肌特异性表达基因MyoG启动子的克隆及应用
摘要：
本发明属于动物基因工程技术领域，具体涉及一种猪的骨骼肌特异性表达基因MyoG启动子活性区域的分离鉴定和功能验证。从猪的基因组中克隆了骨骼肌特异表达基因MyoG上游806bp启动子，其核苷酸序列如SEQ ID NO：1所示。结果表明本发明全长为806bp的启动子片段具有独立的启动活性兼肌肉组织特异性。利用MyoG活性启动子序列驱动外源基因PPARγ的表达，并可用于制备转基因小鼠。本发明还公开了所述启动子片段的制备方法、双荧光素酶活性检测系统、定量PCR和Western Blot方法对启动子活性分析的应用。
摘要（英）：

The invention belongs to the technical field of animal gene engineering, and particularly relates to separation identification and functional verification of active regions of a porcine skeletal muscle specific expression gene MyoG promoter. The skeletal muscle specific expression gene MyoG forward 806bp promoter is cloned from the porcine genome, and the nucleotide sequence is disclosed as SEQ ID NO:1. The result proves that the promoter segment which is 806bp long has independent starting activity and muscle tissue specificity. The MyoG active promoter sequence can be used for driving the expression of the exogenous gene PPAR gamma, and can also be used for preparing a transgenic mouse. The invention also relates to a preparation method of the promoter segment, a dual-luciferase activity detection system, and application of a quantitative PCR (polymerase chain reaction) and Western Blot method in promoter activity analysis.

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