利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN104059977A 一种沙门氏菌血清型鉴定方法及其试剂盒 有权

一种沙门氏菌血清型鉴定方法及其试剂盒
摘要：
本发明提供一种沙门氏菌血清型鉴定方法，包括：1)提取待测样品中的DNA；2)将步骤1)获得的DNA作为模板，进行PCR检测，其中，PCR检测靶标为沙门氏菌CRISPR1和CRISPR2；3)将步骤2)中的PCR产物进行序列测定；4)借助在线免费软件CRISPRfinder，寻找对应CRISPR的前三个间隔序列，并与各血清型标准间隔序列进行同源比对；5)比对结果的判断，可同时鉴定23种沙门氏菌常见血清型。本发明还提供了一种沙门氏菌血清型的鉴定试剂盒，所述试剂盒包括用于扩增沙门氏菌CRISPR1和CRISPR2的引物，并提供23种沙门氏菌常见血清型的标准间隔序列表。
摘要（英）：

The invention provides a salmonella serotype identification method, comprising the following steps: 1) extracting a deoxyribonucleic acid (DNA) in a to-be-tested sample; 2) taking the DNA obtained in the step 1) as a template, and carrying out polymerase chain reaction (PCR) detection, wherein the PCR detection target is salmonella CRISPR1 and CRISPR2; 3) carrying out sequence determination on a PCR product in the step 2); 4) searching front three spacer sequences corresponding to the CRISPRs by virtue of online free software CRISPRfinder and carrying out homologous comparison with each serotype standard spacer sequence; and 5) judging the comparison results, and simultaneously identifying 23 common salmonella serotypes. The invention also provides a kit for identifying the salmonella serotypes. The kit comprises a primer for amplifying the salmonellae CRISPR1 and CRISPR2, and standard spacer sequence tablets of 23 common salmonella serotypes are provided.

公开/授权文献
0/0